Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
نویسندگان
چکیده
Functionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were haracterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to evelop an alcohol oxidase (AlcOx) biosensor, in which AlcOx–BSA was cross-linked with glutaraldehyde and attached by drop-coating. The xperimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at −0.3 V s. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained as 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT. 2008 Elsevier Ltd. All rights reserved.
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